院重点实验室硕士生梁小龙在《RSC Advances》期刊上正式发表Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery一文。本研究受国家863项目（No. 2013AA064402）和国家自然科学基金（31570121）等项目资助。

文章索引/Citation：

Xiaolong Liang^†, Rongjiu Shi^†, Mark Radosevich, Feng Zhao, Yingyue zhang, Siqin Han, Ying Zhang*. 2017. Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery. RSC advances, 7: 20667-20676. (SCIⅡ区，IF 3.108).

文章摘要：Bacillus mojavensis JF-2 produces water-soluble lipopeptide under aerobic conditions, while Pseudomonas stutzeri DQ1 grows rapidly under anaerobic conditions. These bacteria were used to construct an engineered strain for anaerobic lipopeptide production by protoplast fusion for potential use in microbially enhanced oil recovery (MEOR). The resulting fusant strain FA-2 produced lipopeptide (382 mg/l) anaerobically at temperatures up to 50℃, across a pH range of 4.5-10.0, and at salt concentrations as high as 10% NaCl. Experimental results from a physical simulation core at 39℃ suggest that FA-2 has potential for use in MEOR.